U.S. Customs and Border Protection announced the seizure Thursday, saying a January 11 search turned up more than 3,800 pounds of meth, 56 pounds of cocaine and 11 pounds of heroin.
In a statement Thursday, Customs and Border Protection officials confirmed almost two tons of meth were confiscated in Los Angeles last month as it was bound for Australia.
Video released by the AFP shows a number of boxes containing large white bricks of the drug, and three people being taken into custody.
The drugs were detected by United States authorities on January 9 with 25kgs of cocaine and 5kgs of heroin also located.
The Australian Federal Police said the Victorian Joint Organized Crime Task Force was tipped off about a large-scale drug shipment from a suspected syndicate in California.
Four people have been arrested in Victoria including an American man and woman, while two others have been arrested in New South Wales.
Authorities in Canada also conducted searches in Burnaby, British Columbia, as they attempted to crack down on the global crime syndicate believed behind the smuggling.
Three fronted court on Friday on charges including attempted drug importation and possession.
Australian Federal Police Assistant Commissioner Bruce Hill said: 'They have been sending smaller amounts over the years.
James Carouso, the US acting ambassador to Australia, said in a statement that "this historic seizure highlights just how important the US-Australian partnership is in protecting Americans and Australians alike".
"The cartel is among one of the most powerful and violent drug trafficking syndicates in the world". We now believe that Mexican cartels are actively targeting Australia'.
Jason Halls, Victoria manager for the Australian Criminal Intelligence Commission, said the drugs would have had an "immeasurable" impact on the Australian community.
The previous record of seized methylamphetamine bound for Australia was 1.3 tonnes found in Western Australia in December 2017.