She was unphased by facing her idol in the women's final at Flushing Meadows, in front of a crowd that was overwhelmingly partial to the 36-year-old Williams - who lost her cool during the match and repeatedly confronted the umpire after she was given multiple code violations.
However some Twitter users weren't so quick to forget the reason for the cartoon in the first place.
Williams accused Ramos of sexism after the match and pointed out that male players have been more aggressive verbally with umpires than she was, but had been not been penalized the same way. "Will the rules change in Serena's matches?".
At Flushing Meadows on Saturday, the 20-year-old was reduced to tears during the presentation ceremony but on her arrival back in Japan on Thursday, she said she had not been saddened by the incident.
Tokyo will host the Summer Olympics for the second time from July 24 to August 9, 2020, and the tennis competition will be held on hard courts - the same surface as the U.S. Open. I asked my daughter learn from it, ' another added.
Rory Carroll reported this story for Reuters News Agency.
The Japanese player's breakthrough triumph in NY was overshadowed by an explosive row between her opponent Williams and umpire Carlos Ramos which resulted in the 23-times Grand Slam champion being docked a game and fined $17,000. Calling him a "thief" reportedly earned her a full game penalty.
The cartoon took away from the original scandal that erupted around Williams' behaviour during the most recent US Open women's final. "So, I was really happy that she said that", Osaka told Ellen DeGeneres."At the time I did think they were booing at me".
"Ramos is tough, one of the best umpires in the world", Strycova said. "I don't cheat to win - I'd rather lose", she said.
Adams came under fire in some circles for inflaming the sensitive situation when she released a statement hours after the final supporting Serena's protest, labelling Serena a "true champion" who "showed a great deal of class and sportsmanship".
Many people, looking at Serena Williams's actions during the US Open women's final last week, remembered of John McEnroe who often argued with umpires during his career.
"I think the umpire did what was within his rights", Murray told BBC Sport.
She said Williams" outburst "poisoned the atmosphere' for her opponent Naomi Osaka.