The UAE's civil aviation authority GCCA has informed its Qatari counterpart entity that a second Emirati commercial airliner was intercepted by Qatari fighter jets while the aircraft was on a "regular scheduled and well-known journey".
GCAA described the incident as unsafe and a violation of global laws.
After Saudi Arabia, the UAE, Bahrain and Egypt imposed a land, sea and air blockade on Qatar in June, Sheikh Abdullah appeared frequently on Saudi and UAE television programmes expressing his views in support of the measures against Doha.
Earlier in day, a first Emirates passenger flight was intercepted by Qatari military jets, said the UAE, which called the action a "flagrant and serious threat to the safety of civil aviation", according to WAM.
The video of Sheikh Abdullah bin Ali al-Thani, a little-known royal family member until the boycott of Qatar by four Arab nations, offered new fuel to the months-long stalemated crisis.
"I am a guest of Sheikh Mohammed (UAE crown prince), but it is not hosting now, it is now an imprisonment", Sheikh Abdullah said. "Sheikh Mohammed bears full responsibility for anything that happens to me".
Qatar has vehemently denied the allegations and demands.
She said: "It seems that the Civil Aviation Authority in the UAE, by publishing false claims, is trying to pre-empt events and cover over news of the Emirati plane penetration of Qatari airspace".
Qatar's foreign minister says Gulf crisis is all over a woman Qatar's Foreign Minister has made the extraordinary claim that the diplomatic crisis in the gulf was all brought about by a dispute over a woman renewing her passport. A Qatari government official dismissed the report as "completely untrue".
Ali Rashid al-Nuaimi, who heads Abu Dhabi's education department and a counter-extremism centre, sent a series of tweets saying Sheikh Abdullah is free to go "whenever he wants".
"Despite that, the State of Qatar principally stands with the protection of rights for every individual and affirms the entitlement of his family to pursue all legal means for the protection of his rights".
Sheikh Abdullah bin Ali is the second son of the late Emir Ali bin Abdallah al-Thani who ruled from 1949 to 1960.
"The Qatari complaint about the UAE's violation of its airspace is incorrect, and we are working on the official response with evidence".