An Uber spokesman said: "While we have today filed our appeal so that Londoners can continue using our app, we hope to continue having constructive discussions with Transport for London".
Uber on Friday, hours before the deadline, filed papers with Westminster magistrates court, an appeals process that will allow it to continue operating London. The first hearing on the issue is expected on December 11.
Today was the last day Uber could appeal TfL's decision to renew its licence and now in the hands of the courts, Londoners could see Uber stick around in the future. "We are determined to make things right".
Uber Technologies Inc. appealed a London regulator's decision to revoke the vehicle service's license as it seeks more settlement talks.
TfL has highlighted the company's approach to reporting serious criminal offences and to obtaining criminal record checks for drivers as factors in its decision.
New global Chief Executive Dara Khosrowshahi met TfL Commissioner Mike Brown for talks earlier this month, which both sides said were "constructive" as the $70-billion firm tries to fix its relationship with the regulator.
Earlier this week, London mayor Sadiq Khan said the appeal against Transport for London "will take its course".
The new boss has apologised for "the mistakes we've made" and accepted that the company had "got things wrong".
His predecessor, Travis Kalanick, left the company following allegations that managers didn't adequately address reports of sexual harassment.
Uber has been used by 3.5 million Londoners, developing a loyal fan base by offering convenience and lower prices than traditional "Black Cabs".
More than 850,000 people have signed an online petition launched by Uber, urging TfL to reverse its ruling.